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PCR Kit "Shikajenius PCR Kit for Diarrheagenic Escherichia coli 2"

Simultaneous detection of the main five types of diarrheagenic Escherichia coli and the pathogenic genes related to E. albertii using multiplex PCR method.

This product can simultaneously detect pathogenic genes related to five main types of enteropathogenic Escherichia coli (E. coli) using multiplex PCR: EHEC/VTEC (enterohemorrhagic/verotoxin-producing E. coli), EPEC (enteropathogenic E. coli), ETEC (enterotoxigenic E. coli), EIEC (enteroinvasive E. coli), EAggEC (enteroaggregative E. coli), and E. albertii. **Features** - Using two types of primer mixes, it can simultaneously and distinctly detect species-specific genes of E. coli and E. albertii along with seven types of pathogenic factors*1. (*1 Pathogenic factors: Verotoxin types 1 and 2 (VT1, 2) genes (stx1, 2), heat-stable enterotoxin (ST) gene (est), heat-labile enterotoxin (LT) gene (elt), intimin gene (eae), aggregative adherence factor (aggR), enteroinvasive factor (invE)) - By quantifying the detection results, the judgment results can be clarified. - Reagents necessary for PCR, including positive controls, are included. - The detection results of the internal control can confirm the presence or absence of PCR inhibition.